Stable Cell Line Construction Service

    Alpha Lifetech Inc. is experienced in cell line construction. We are committed to providing high-quality stable cell line construction services to customers worldwide. Our services are proven and trusted.

Stable cell lines are widely used in various research applications. Cell lines can maintain expression levels during a prolonged culture process. Currently, laboratory technologies of maintaining live cell lines separated from their original tissue source have become robust, such as primary cell and recombinant cell culture, but the stability of those cell lines to produce a high level of bioproduct can be a blockbuster. The opportunity exists to explore new technologies to increase clonal stability while minimizing clonal variation. Factors that contribute to instability include gene copy number, position effect, insertion mutagenesis, post-transcriptional and post-translational modifications.

Based on the cDNA library and gene synthesis technology, we provide a fast and convenient method for screening or amplifying genes of interest (GOI). In general, in order to develop a stable cell line, we need to ligate the GOI to the multiple cloning sites (MCS) which under the control of a single or double promoters and then integrate it into the host chromosomes to retain intact host genetic materials in continuous cell culture or just stay as episomal replicants (transient expression cell line). Stable cell line is the preferred solution, not only to produce recombinant protein and antibody, but also for application in small molecule screenings, gene functional studies etc.

Although the construction of stable cell lines is a laborious and time-consuming job, Alpha Lifetech Inc. is able to free customers from this tedious work and allow them to concentrate on more challenging tasks by providing the relevant services. Our scientists have extensive experience in the field of cell culture and cell line generation. We have developed several high-quality stable cell lines and produced many antibodies and proteins through stable cell lines. Our specific biosimilar cell lines and hybridoma cell lines are available in-house, and we are also pleased to provide custom strains to meet your specific requirements.

Inhibitor Library Service

 The experts at Alpha Lifetech Inc. have extensive research experience in the R&D of pharmaceutical compounds. Feasible advice and customized services are provided to support your research.

Small Molecule Inhibitor Libraries Services - Custom Inhibitor Library Service

Alpha Lifetech Inc. collects 1,695 different small molecule inhibitors covering targets including PI3K, VEGFR, RTK pathway, HDAC, Aurora Kinase, CDK and Cell Cycle regulators, Integrase/Proteasome and Phosphatase which consist of a database of inhibitor chemicals for research purposes. Compounds, quantities, format (dry/solid, DMSO or other buffer), and plate layout (Such as Cell Signaling Pathway, Clinical Research, and Targets) are selectable to satisfy your specific requirements of high-through small molecule screening. Simply send us the information of your target of interest and our technical support team will provide a detailed quote accordingly.

-- Container: 96-well format sample storage tube with screw cap and optional 2D barcode -- Size: Customized (50 – 500μL/well; 100μL/well) -- Concentration: Customized (5 – 50 mM/well; 10 mM/well)

Service Features of Custom Inhibitor Library Service

-- Bioactivity and safety confirmed by preclinical research and clinical trials

-- 5 – 50 mM (final concentration) in each well of a 96-well plate

-- Quality Control: NMR, HPLC, LC/MS

-- Documents: Plate layout, IC50

-- Turnaround time: 3-4 weeks 

 

Please download Inhibitor Library (IL) and customize your libraries (or purchase the pre-made libraries). If you have any questions, please feel free to contact us for more support. 

Alpha Lifetech Custom Polyclonal Antibody Production Service

    Alpha Lifetech Inc.'s custom antibody development services include monoclonal and polyclonal antibody generation, production, and modification services. We provide a wide range of polyclonal antibody production in different species including New Zealand white rabbits, goats, sheep, guinea pigs, chickens, rats, mice, and donkeys. 

Antibody Production Service - Custom Polyclonal Antibody Production

    Our custom antibody production services enable you to track and manage your antibody development easily and efficiently. Our well-established antibody platform could provide all the steps of the service: antigen production, antibody generation, purification, and conjugation, screening, and titer analysis. Large-scale antibody production is also available for assay manufacturing.

Custom antibody generation workflow is shown below:

 

    Each step is optional, as we offer a truly custom antibody service and strive to meet all your needs.

Antigen Preparation: antigen design, peptide synthesis, hapten/small molecules modification (KLH, BSA, OVA, Hydralink-conjugated), other antigens like viruses, bacterium, and whole cells preparation.

Antibody Production: Multiple hosts are available, and we offer variable immunization protocols and inject animals with different adjuvants to produce desirable immune reactions.

Antibody Purification: Multiple antibody purification protocols are available: protein A and protein G affinity purification, ion exchange, and peptide affinity (ligand affinity purification).

Antibody Modification: HRP, AP, other fluorescence groups, agarose beads, quantum dots, and magnetic beads conjugation.

 

Features of Custom Polyclonal Antibody Production

 

-- High-quality polyclonal antibodies to tailor your requirements

-- Opportunities for you to perform sample testing at various stages

-- Excellent document support system and strict QC & QA

-- Multiple hosts: goat, sheep, canine, chicken, goose, pig, ferret, alligator, bovine, monkey, donkey, horse, ostrich, hamster, rat, and turkey.

Alpha Lifetech provides Custom Rat Monoclonal Antibody Service

    Alpha Lifetech Inc. is capable of providing the whole service of murine hybridoma to worldwide scientists. These services include antigen design/synthesis, animal immunization, cell fusion and hybridoma selection/characteristics, with which we can specialize each project.

Hybridoma Production – Rat Hybridoma & Monoclonal Antibodies

 

Rats have some intrinsic characteristics covering that rats have better immune response than mouse under certain antigen stimulation, especially proteins derive from human beings, because of these proteins from human have a higher homology with mouse. In this case, rat is regarded as a suitable host for hybridoma/monoclonal antibody production and a tool for yielding higher specificity and affinity monoclonal antibodies. Meanwhile, rats have a higher capacity of 50-100 ml ascites of each rat compared with 3-5 ml ascites of each mouse which means scientists could harvest monoclonal antibodies from the rat hybridoma not only with a big amount but also with a batch consistency.

Alpha Lifetech Inc. has established a unique hybridoma platform to develop high-affinity monoclonal antibodies in rats. We use Winstar strain of rats as a resource of spleen cells and IR 983F myeloma as a chaperone cell to create high-expression and high-affinity monoclonal antibodies. Compared with commonly used mouse antibodies, rat antibodies do not have the background cross-reaction problems in immune-detection of antigens out of a mouse background, such as a mouse antigen from a mouse animal model. Another reason is that antibodies against mouse protein are difficult to be raised in mice themselves. Furthermore, we have created IR 983F cell line with lower expression of α-1, 6-fucosyltransferase by using RNAi technology, because IR 938F as chaperone cell possessing lower level of α-1, 6-fucosyltransferase that could efficiently reduce glycosylation of antibody (similar to function of YB2/0 myeloma cell line fused with B lymphocytes). Therefore, antibodies from other species have to be considered and rat monoclonal antibodies are a good choice for yielding low glycol modification antibodies.

 

 

Program Features of Rat Hybridoma & Monoclonal Antibodies

 

-- Picomolar affinity and high specificity

-- Ability to recognize subtle changes such as post-translational modifications

-- Novel epitope recognition

-- Good resource for diabody production

-- Cross-reactive antibodies recognizing human proteins and mouse orthologs

-- Excellent IHC results with formalin fixed paraffin embedded tissue

-- Ability to generate large panels of bioactive antibodies

-- Idea antibody candidates for ADCC

The construction of hybridoma cell lines is a laborious and time-consuming job, we hope to free customers from this tedious work to a more challenge work by using our relevant services. Experts in Alpha Lifetech Inc. have extensive experience in the field of cell culture and cell line generation. We have developed several perfect stable cell lines and also produced a large number of antibodies and proteins through stable cell lines. Our specific biosimilar cell lines and hybridoma cell lines are available in-house, and we are also pleased to provide custom strains to meet our clients' individual requirement.

If you have any questions, please feel free to contact us at any time.

Alpha Lifetech Inc. offers rabbit monoclonal antibodies

 In the realm of biotechnology and scientific research, obtaining high-quality antibodies is essential for accurate and reliable results. Alpha Lifetech Inc. has developed a groundbreaking monoclonal antibody technology specifically tailored for rabbit monoclonal antibody production.

Rabbit monoclonal antibodies have gained significant attention in recent years due to their unique characteristics and advantages over traditional mouse monoclonal antibodies.

 

The Key Benefits of Rabbit Monoclonal Antibodies:

 

Enhanced Sensitivity and Specificity: Rabbit monoclonal antibodies are known for their high specificity and sensitivity, enabling them to recognize and bind their target antigens with greater precision. This makes them particularly valuable in various applications, such as diagnostic tests and research purposes. 

Broadened Epitope Recognition: Rabbit immune systems generate a broader and more diverse range of antibodies compared to mice. This diversity allows rabbit monoclonal antibodies to recognize a wider variety of antigenic epitopes, including those that may be poorly immunogenic in mice.

Improved Stability and Affinity: Rabbit monoclonal antibodies typically exhibit better stability and affinity towards their target antigens than mouse-derived antibodies. This improved affinity and stability can lead to enhanced performance in various applications, including immunoassays, flow cytometry, and immunohistochemistry.

Faster Development and Production: Alpha Lifetech Inc.'s cutting-edge technology streamlines the process of rabbit monoclonal antibody production, enabling researchers to obtain high-quality monoclonal antibodies more quickly and efficiently.

At Alpha Lifetech Inc., we pride ourselves on offering top-quality rabbit monoclonal antibodies tailored to meet the specific needs of our clients. Alpha Lifetech Inc. can offer thousands of rabbit monoclonal antibodies against various biomolecules to satisfy your requirements, based on cell fusion technology, phage display technology, and single B cell screening technology. Our expert team of scientists utilizes cutting-edge technologies and advanced techniques to generate highly specific and high-affinity rabbit monoclonal antibodies. We offer a comprehensive catalog of antibodies for various targets and research applications and customized services to develop and optimize antibodies according to your unique requirements.

Whether you're investigating novel targets, developing diagnostic assays, or studying complex biological processes, Alpha Lifetech Inc. is your trusted partner for exceptional rabbit monoclonal antibodies! For more information, please contact us and our technical support team will get back to you as soon as possible.

Monoclonal Antibodies

    Monoclonal antibodies (mAbs) are immunoglobulins designed to target a specific epitope on an antigen. The antigen-binding region of the mAb is formed by the variable domains of the heavy and light chains and contains the complementarity-determining region that imparts high specificity for the target antigen. The monoclonal antibodies (mAb or moAb) recognize only one epitope per antigen and are less likely than polyclonal antibodies to react against other proteins. Monoclonal antibodies can be used to identify specific antigens. Due to their essential functions in various areas, monoclonal antibodies have become popular molecules.

    The first monoclonal antibody was generated in mice in 1975 by Milstein and Kohler using the hybridoma technique.

    The most popular approach for creating monoclonal antibodies is still hybridoma production. The hybridomas that produce monoclonal antibodies from the stable fusions to develop in vitro are chosen, sub-cloned, and formed. These clones are then cultivated, and either the cells themselves or the lysates from the cell culture are used to purify the antibodies they make. Each monoclonal antibody's specificity is determined by its binding site on the target protein.

Monoclonal antibodies (mAbs) production (Polyclonal and Monoclonal Antibody Production | Microbiology | | Course Hero)

    Alternative techniques for creating monoclonal antibodies have become available. The so-called "phage-display method" allowed for the specificity screening of recombinant antigen-binding antibodies from a library of fusions to a carrier phage protein. The constant region of recombinant antibodies may now be tailored due to significant advancements in their manufacture, leading to cell lines that can produce them. The benefit of this novel manufacturing approach is that it avoids the problems associated with a hybridoma's long-term stability, including the eventual loss of a priceless clone.

Advantages of Monoclonal Antibodies

(1) Batch to batch reproducibility

(2) The possibility of producing large amounts of identical antibodies

(3) Highly specific to a unique epitope

(4) Low cross-reactivity

(5) Most sensitive in tests where protein levels need to be quantified

 

Alpha Lifetech Inc. provides highly validated monoclonal antibodies to support your research. We offer thousands of mouse monoclonal antibodies, rabbit monoclonal antibodies， and over 700 high-quality recombinantly expressed biosimilar antibodies. The antibodies undergo comprehensive validation testing to ensure high quality, high consistency, and specific binding to the molecule targeted and approved for your research use. Based on our professional antibody R&D team and rich experience in antibody production, we are confident in the quality of our antibody products and strive to accelerate your research. The mature technical route and dependable quality will save you time.

    Whether you are ordering our antibodies for your research use or talking to one of our technical service representatives, our staff is dedicated to providing professional support. Please feel free to contact us for more information about our products and related promotions.